anti pde10a  (R&D Systems)

Journal: International Journal of Neuropsychopharmacology

Article Title: Identification of Phosphodiesterase-7A (PDE7A) as a Novel Target for Reducing Ethanol Consumption in Mice

doi: 10.1093/ijnp/pyae032

Figure Lengend Snippet: PDE7A expression was upregulated following ethanol administration in female mice. (A–C) Protein expression of PDE isoforms, including PDE1B, PDE2A, PDE4A, PDE4B, PDE4D, PDE7A, PDE7B, PDE9A, and PDE10A, in the hippocampus, prefrontal cortex, and striatum of C57BL/6J female mice after saline or EtOH (i.p., 2.5 g/kg) injection for 7 days. The upper image shows the true bands of PDE7A and GAPDH at their respective KDa quantitative value ( t test, PDE7A: EtOH vs saline, **** P < .0001). (D–F) Expression of PDE isoforms in male mice after EtOH injection. The upper image shows the true bands of PDE7A and GAPDH at their respective KDa quantitative value ( t test, PDE7A: EtOH vs saline, P > .05). N = 6 mice for each group. All data shown are means ± SEM. The ratio of PDE7A to the respective GAPDH in each lane was calculated for statistical analysis.

Article Snippet: The antibodies against PDE1B (Cat# ab182565, 1:1000), PDE2A (Cat# ab140650, 1:1000), PDE4A (Cat# ab200383, 1:1000), PDE4B (Cat# ab170939, 1:1000), PDE4D (Cat# ab171750, 1:1000), PDE7B (Cat# ab170914, 1:1000), PDE10A (Cat# ab227829, 1:1000), or PKA (Cat# ab32514, 1:1000) were purchased from Abcam (Cambridge, UK).

Techniques: Expressing, Saline, Injection